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Paul Shashkov
Paul Shashkov

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A key module of the IceBear software is the drop viewer (Fig. 6). The pages of the drop viewer can be reached in several ways and it has five main functions (Fig. 6). (i) Measuring and scoring, allowing fast hands-free viewing of all images of a plate along with scoring and (where scale information is provided by the imager) measurement of the size of the crystal. (ii) Viewing the imaging history, where the change in the drop contents over time can be observed as a movie displaying the same drop as recorded in different inspections (Supplementary Fig. S2a). (iii) Showing crystallization conditions and other key information (Fig. 7a). (iv) Crystal selection, allowing crystals to be marked for onward processing. (v) Navigation, showing a plate overview that can be toggled between visible and ultraviolet images, along with side-by-side comparison of all subpositions within a well (Supplementary Fig. S2b). Keyboard shortcuts throughout enable rapid interaction.


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